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Home Antibody All anti-SQSTM1 antibodies

Anti-SQSTM1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300177
  • Rabbit Polyclonal Antibody against SQSTM1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401285) , 20ug Explanation
100ug 325 In Stock
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WB(2)
IHC(1)
IF(2)
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Also for SQSTM1 (NM_003900)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:50~100, WB: 1:1000, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens sequestosome 1 (SQSTM1), transcript variant 1
Alternative NameA170; OSIL; p60; p62; p62B; PDB3; ZIP3
Database LinkNP_003891
Entrez Gene 8878 Human
Entrez Gene 18412 Mouse
Entrez Gene 113894 Rat
FunctionSQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of SQSTM1 Antibody (C331) Pab (Cat.#TA300177) pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified Pab.
WB Image
Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ) (Cat.#TA300177). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF Image
Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (Cat# TA300177) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
IF Image
Fluorescent image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA300177 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).

 

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