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Home Antibody All anti-PIM2 antibodies

Anti-PIM2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300051
  • PIM2 Antibody (C-term ) Rabbit Polyclonal Antibody (Pab)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416355) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)
IP(1)
IHC(1)
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Also for PIM2 (NM_006875)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.
Clone Name IsotypeIg
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:1000, IP: 1:50~100, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Pim-2 proto-oncogene, serine/threonine kinase (PIM2)
Alternative Name
Database LinkNP_006866
Entrez Gene 11040 Human
FunctionPim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.
Related PathwayProtein KinaseDruggable Genome Acute myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-PIM2 Antibody (C-term) (Cat.#TA300051) in Hela cell line lysates (35ug/lane). PIM2 (arrow) was detected using the purified Pab.
WB Image
Western blot analysis of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292) (Cat.#TA300051). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IP Image
PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.

 

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