Glycogen synthases kinase 3 alpha (GSK3-a) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-a activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-a by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-a activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).
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HEK293T cells were transfected with the pCMV6-XL5 control or pCMV-GSK3alpha (SC112599) cDNA for 72 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSK3alpha Rabbit mAb. The size markers are shown in numbers and the correct 51kDa GSK3alpha protein is indicated by an arrow.
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