OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-c-JUN antibodies

Anti-c-JUN Antibody E254

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300032
  • Rabbit Monoclonal Antibody against c-JUN (N-term)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400825) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IP(1)
IHC(1)
IF(2)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen. Predicted to cross-react with pig, based on sequence homology.
Clone NameE254 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF, IP Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250; ICC: 1:250; IP: Use a concentration of 5 ug/ml
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens jun proto-oncogene (JUN)
Alternative NameAP-1; AP1; c-Jun
Database LinkNP_002219
Functionc-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - c-Jun antibody [E254]; Anti-c-Jun [E254] antibody at 1/2000 dilution + NIH 3T3 cell lysate.Predicted band size : 36 kDa.Observed band size : 40 kDa .
Assay Image
Other-Anti-c-Jun antibody [E254](TA300032); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - c-Jun antibody [E254]; Immunohistochemical analysis of cJun expression in paraffin embedded skin carcinoma tissue sample, using 1/250 TA300032.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [E254]; ICC/IF image of TA300032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [E254]; TA300032 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) , by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.The cells were incubated at 37°C for 4h in media containing different concentrations of (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300032 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IP Image
Immunoprecipitation - Anti-c-Jun antibody [E254]; c-Jun was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to c-Jun and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300032.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 45kDa; c-Jun

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title