Home Antibody All anti-BLK antibodies
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the sequence of human BLK within aa 1-100.|
||0.5~1.0 mg/ml (Lot Dependent)
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Homo sapiens B lymphoid tyrosine kinase (BLK)|
|Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.|
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HEK293T cells were transfected with the pCMV6-XL5 control or pCMV-BLK (SC119056) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BLK Rabbit polyclonal Ab. The size markers are shown in numbers and the correct 57 kDa BLK protein is indicated by an arrow.