Anti-c-Myc Antibody,

Also for c-Myc (NM_002467)

OriGene Data

Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ
Clone Name		Isotype	IgG
Species Reactivity	Human, Mouse	Concentration
Guaranteed Application *	WB, IF	Suggested Dilutions	WB: 1: 1,000; ICC: 1:50-100; IP: 1: 100
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)
Alternative Name	bHLHe39; c-Myc; MRTL
Database Link	NP_002458
Function	Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Related Pathway	Apoptosis Cell cycle EGFR1 Signaling Pathway Jak-STAT signaling pathway MAPK signaling pathway TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-XL5 control or pCMV-Myc (SC112715) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myc Rabbit MoAB. The size markers are shown in numbers and the correct 60 kD Myc protein is indicated by an arrow.

WB Image

Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.

IF Image

Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62)

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-c-Myc Phospho Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data