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Anti-c-Myc Phospho Antibody
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Also for c-Myc (NM_002467)
|A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ|
||WB: 1: 1,000; ICC: 1:50-100; IP: 1: 100
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)|
|bHLHe39; c-Myc; MRTL|
|Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3). |
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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HEK293T cells were transfected with the pCMV6-XL5 control or pCMV-Myc (SC112715) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myc Rabbit MoAB. The size markers are shown in numbers and the correct 60 kD Myc protein is indicated by an arrow.
Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62)