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Anti-IdU Antibody Clone 2B10

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA190129
  • IdU mouse monoclonal antibody,clone 2B10
100ul 325 In Stock
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IHC(3)
IF(1)

OriGene Data

ImmunogenIododeoxyuridine coupled to keyhole limpet hemocyanin.
Clone NameClone 2B10 IsotypeIgG2b
Species Reactivity Concentration4.54mg/ml
Guaranteed Application *IHC, IF Suggested Dilutions IHC 1:150, IF 1:150,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography
Note 5-chloro-2'-deoxyuridine (ClDU), 5-bromo-2'-deoxyuridine (BrdU), 5-iodo-2'-deoxyuridine (IdU) and 5-ethynyl-2'-deoxyuridine (EdU) are nucleoside analogs of thymidine. Cells that treated with these analogs will incorporate the chemicals into the genomic DNA during S-phase. Immunochemical method detection of these analogs is thus used to quantify the cell proliferation, cell cycle status in vitro or in vivo. Since the thymidine analogs can be passed onto the daughter cells, they can also used to trace dividing cell fate in a short period of time over 3 generations. In addition, combination of different analogs and their specific antibodies can be used to trace cell fate in different time frames.

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IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue from BrdU injected mouse using anti-IDU mouse monoclonal antibody.(TA190129)
IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue from IDU injected mouse using anti-IDU mouse monoclonal antibody.(TA190129)
IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue from CLDU injected mouse using anti-IDU mouse monoclonal antibody.(TA190129)
IF Image
Immunocytochemistry staining of HT-29 cells pulsed with 5-iodo-2'-deoxyuridine (IdU)using mouse monoclonal anti-IDU antibody (1:150)

 

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