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Anti-SYK PHOSPHO Antibody EP573-4
|A phospho specific peptide corresponding to residues surround Tyrosine 323 of human Syk was used as an immunogen. This antibody detects Syk phosphorylated at Tyrosine 323.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens spleen tyrosine kinase (SYK), transcript variant 1|
Entrez Gene 6850 Human
|Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Syk (pY323) was found to be critical for the enrichment of the catalytic subunit p110delta of PI3K class I(A) as well as for the generation of PI3K products at the leading edge of the majority of polarized cells (6). |
|Protein KinaseDruggable Genome Natural killer cell mediated cytotoxicityB cell receptor signaling pathwayFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosis|
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Western blot - Syk (phospho Y323) antibody [EP573Y]; All lanes : Anti-Syk (phospho Y323) antibody [EP573-4] at 1/2000 dilution.Lane 1 : untreated U937 cell lysates.Lane 2 : U937 cell lysates treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit, HRP labeled, at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Syk (phospho Y323) antibody [EP573Y]; Immunohistochemical analysis of Syk (phospho Y323) expression in paraffin embedded human lymph node using 1/100 TA301048.