Anti-SUPT5H Antibody, EPR5145(2)

Also for SUPT5H (NM_001111020)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues in human SUPT5H was used as an immunogen.
Clone Name	EPR5145(2)	Isotype
Species Reactivity	Human, Mouse, Rat	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1:1,000 - 10,000;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2
Alternative Name	SPT5; SPT5H; Tat-CT1
Database Link	NP_001104490
Function	SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Related Pathway

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WB Image

Western blot analysis on (A) HeLa, (B) HepG2, (C) RAW 264.7, (D) PC-12, (E) NIH/3T3, (F) Jurkat, and (G) 293T cell lysates using anti-SUPT5H RabMAb.

IHC Image

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUPT5H RabMAb.

IF Image

Immunofluorescent staining of HeLa cells using anti-SUPT5H RabMAb.

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Anti-SUPT5H Antibody EPR5145(2)

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data