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Home Antibody All anti-SUPT5H antibodies

Anti-SUPT5H Antibody EPR5145(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310887
  • Rabbit Monoclonal antibody against SUPT5H
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
Assay(1)
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Also for SUPT5H (NM_001111020)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SUPT5H was used as an immunogen.
Clone NameEPR5145(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2
Alternative NameSPT5; SPT5H; Tat-CT1
Database LinkNP_001104490
FunctionSUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Related Pathway

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WB Image
Western blot - Anti-SUPT5H antibody [EPR5145(2)]; All lanes : Anti-SUPT5H antibody [EPR5145(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : RAW264.7 cell lysate.Lane 4 : PC12 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lane 6 : Jurkat cell lysate.Lane 7 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 121 kDa.
Assay Image
Other-Anti-SUPT5H antibody [EPR5145(2)](TA310887); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUPT5H antibody [EPR5145(2)]; TA310887 at 1/100 dilution staining SUPT5H in paraffin-embedded Human colon tissue by immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-SUPT5H antibody [EPR5145(2)]; TA310887 at 1/100 dilution staining SUPT5H in HeLa cells by immunofluorescence.

 

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