Home All anti-SUPT5H antibodies
Anti-SUPT5H Antibody EPR5145(2)
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for SUPT5H (NM_001111020)
|A synthetic peptide corresponding to residues in human SUPT5H was used as an immunogen.|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:1,000 - 10,000;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2|
|SPT5; SPT5H; Tat-CT1|
|SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.|
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis on (A) HeLa, (B) HepG2, (C) RAW 264.7, (D) PC-12, (E) NIH/3T3, (F) Jurkat, and (G) 293T cell lysates using anti-SUPT5H RabMAb.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUPT5H RabMAb.
Immunofluorescent staining of HeLa cells using anti-SUPT5H RabMAb.