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Anti-STAT5A Antibody E289
Also for STAT5A (NM_003152)
|A synthetic peptide corresponding to residues in C-terminus of human Stat5a was used as immunogen. The antibody does not cross-react with other Stat family members.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100; FC: 1:10; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens signal transducer and activator of transcription 5A (STAT5A)|
|Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3).|
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
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Western blot - STAT5a antibody [E289]; Anti-STAT5a antibody [E289] at 1/1000 dilution + A431 cell lysate.Predicted band size : 91 kDa.Observed band size : 92 kDa .
Western blot ; Anti-STAT5a antibody [E289] at 1/1000 dilution + Human STAT5a full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 20 seconds
Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289]; ab32043 at 1/250 dilution, staining human squamous lung carcinoma by Immunohistochemistry, paraffin-embedded tissue.
Flow Cytometry-Anti-STAT5a antibody [E289](ab32043); Overlay histogram showing Jurkat cells stained with ab32043 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.