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Anti-STAT1 PHOSPHO Antibody EPR3146
Also for STAT1 (NM_007315)
|A phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant alpha|
|CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91|
Entrez Gene 6772 Human
Entrez Gene 20846 Mouse
Entrez Gene 25124 Rat
|Stat-1 is a latent transcription factor that functions both as a signal transducer in the cytoplasm and an activator of transcription in the nucleus. Stat-1 is one of seven members of the Signal transducer and activators of transcription (STAT) family (1). Stat-1 is activated by a large number of ligands: IFN-alpha, IFN-gamma, EGF, PDGF and IL6 (2-3). IFN-gamma signaling induces phosphorylation of the Stat-1 residue Tyrosine 701, which facilitates dimerization, nuclear translocation, and DNA binding (4). Upon tyrosine phosphorylation, Stat-1 dimerizes with Stat-2 and associates with ISGF3/IR9 to form a complex called the ISGF3 transcription factor (5). This activation subsequently allows the complex to translocate to the nucleus and bind specific enhancer elements. Additional complexes can form upon IFN stimulation (1). Defects in Stat-1 are the cause of Stat-1 deficiency, characterized by mycobacterial or viral diseases (6).|
|Transcription FactorsDruggable Genome Chemokine signaling pathwayToll-like receptor signaling pathwayJak-STAT signaling pathwayPathways in cancerPancreatic cancer|
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Western blot - STAT1 (phospho S727) antibody [EPR3146]; All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase (AP).Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 91 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT1 (phospho S727) antibody [EPR3146]; TA307009, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.