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Home Antibody All anti-STAT1 antibodies

Anti-STAT1 PHOSPHO Antibody EPR3147

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307008
  • Rabbit monoclonal antibody against Stat-1 Phospho (pY701)(clone EPR3147) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
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Also for STAT1 (NM_007315)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Tyrosine 701 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Tyrosine 701.
Clone NameEPR3147 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant alpha
Alternative NameCANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Database LinkNP_009330
Entrez Gene 6772 Human
FunctionStat-1 is a latent transcription factor that functions both as a signal transducer in the cytoplasm and an activator of transcription in the nucleus. Stat-1 is one of seven members of the Signal transducer and activators of transcription (STAT) family (1). Stat-1 is activated by a large number of ligands: IFN-alpha, IFN-gamma, EGF, PDGF and IL6 (2-3). IFN-gamma signaling induces phosphorylation of the Stat-1 residue Tyrosine 701, which facilitates dimerization, nuclear translocation, and DNA binding (4). Upon tyrosine phosphorylation, Stat-1 dimerizes with Stat-2 and associates with ISGF3/IR9 to form a complex called the ISGF3 transcription factor (5). This activation subsequently allows the complex to translocate to the nucleus and bind specific enhancer elements. Additional complexes can form upon IFN stimulation (1). Defects in Stat-1 are the cause of Stat-1 deficiency, characterized by mycobacterial or viral diseases (6).
Related PathwayTranscription FactorsDruggable Genome Chemokine signaling pathwayToll-like receptor signaling pathwayJak-STAT signaling pathwayPathways in cancerPancreatic cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - STAT1 alpha (phospho Y701) antibody [EPR3147]; All lanes : Anti-STAT1 alpha (phospho Y701) antibody [EPR3147] at 1/1000 dilution.Lane 1 : A431 cell lysates untreated with Epidermal Growth Factor (EGF).Lane 2 : A431 cell lysates treated with Epidermal Growth Factor (EGF).Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.

 

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