Invalid object name 'antibody_LocusMapping'. Anti-SSB Antibody, clone 3H9 - OriGene
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Anti-SSB TRUEMAB Antibody clone 3H9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500410
  • anti-SSB mouse monoclonal antibody,clone 3H9
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401091) , 20ug Explanation
100ul 325 In Stock
CF500410
  • Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody,clone 3H9
100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(5)
IF(1)
FC(1)
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Also for SSB (NM_003142)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human SSB expression vector
Clone Nameclone 3H9 IsotypeIgG2a
Species ReactivityHuman , Dog Concentration1.21 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB), transcript variant 1
Alternative NameLa; La/SSB; LARP3
Database LinkNP_003133
FunctionLa is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500410)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500410)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500410)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500410)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500410)
IF Image
Immunofluorescent staining of HT29 cells using anti-SSB mouse monoclonal antibody (TA500410).
IP Image
Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500410),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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