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Anti-SSB TrueMAB Antibody clone 1E11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Protein expressed in 293T cell transfected with human SSB expression vector|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, IP, FC
||WB 1:1000~2000, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB)|
|La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB(RC205013).
Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).
Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500406),(Red), compared to a nonspecific negative control antibody,(Blue).