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Anti-SRF PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human SRF around the phosphorylation site of serine 77 (L-Y-SP-G-S).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|SRF (Phospho-Ser77) antibody detects endogenous levels of SRF only when phosphorylated at serine 77.
|Homo sapiens serum response factor (c-fos serum response element-binding transcription factor) (SRF), transcript variant 1|
MAPK signaling pathway
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Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using SRF (Phospho-Ser77) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody.The picture on the right is treated with the synthesized peptide.