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Anti-SRC PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Src (phospho-Tyr529) antibody detects endogenous levels of Src only when phosphorylated at tyrosine 529.
|Homo sapiens v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (SRC), transcript variant 1|
|ASV; c-SRC; p60-Src; SRC1|
EGFR1 Signaling Pathway
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Western blot analysis of extracts from COS7 cells, using Src (phospho-Tyr529) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Src (phospho- Tyr529) antibody.The picture on the right is treated with the synthesized peptide.