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Anti-SRC PHOSPHO Antibody Y232
|A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 529 of human Src was used as immunogen. The antibody will detect Src phosphorylation on Tyrosine 529 of both isoforms. Predicted to cross-react with mouse (both isoforms), rat and|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, DB
||WB: 1:5,000-10,000; ICC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (SRC), transcript variant 1|
|ASV; c-SRC; p60-Src; SRC1|
|This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. |
EGFR1 Signaling Pathway
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Western blot - Src (phospho Y529) antibody [Y232]; All lanes : Anti-Src (phospho Y529) antibody [Y232] at 1/10000 dilution.Lane 1 : Untreated A431 cells.Lane 2 : EGF treated A431 cells.Lane 3 : EGF and alkaline phosphatase treated A431 cells.Predicted band size : 60 kDa.Observed band size : 55 kDa .
Immunocytochemistry/ Immunofluorescence - Src (phospho Y529) antibody [Y232]; Immunofluorescent analysis of Src (phospho Y529) expression in A431 cells using 1/50 TA303702.
Dot Blot - Src (phospho Y529) antibody [Y232]; Dot Blot analysis on immunogen phospho peptide (A) and non phospho peptide (B) using 1/2000 TA303702.