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Anti-SQSTM1 TRUEMAB Antibody Clone OTI1E9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502127 SQSTM1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) 100ul $325 In Stock
LC401285 SQSTM1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502127 Carrier-free (BSA/glycerol-free) SQSTM1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(1)
FC(1)
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Also for SQSTM1 (NM_003900)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
Clone NameClone OTI1E9 IsotypeIgG2b
Species ReactivityHuman , Rat , Monkey Concentration0.86 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 47.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens sequestosome 1 (SQSTM1), transcript variant 1
Alternative NameA170; OSIL; p60; p62; p62B; PDB3; ZIP3
Database LinkNP_003891
Entrez Gene 8878 Human
Entrez Gene 113894 Rat
Entrez Gene 705481 Monkey
FunctionThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009].
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
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Anti-SQSTM1 mouse monoclonal antibody (TA502127) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
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HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502127), and then analyzed by flow cytometry.
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