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Anti-SQSTM1 Antibody EPR4844
Also for SQSTM1 (NM_003900)
|A synthetic peptide corresponding to residues in human Sequestosome-1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:10000 - 1:50000; ICC: 1:100 - 1:500; FC: 1:10 - 1:100
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Homo sapiens sequestosome 1 (SQSTM1), transcript variant 1|
|A170; OSIL; p60; p62; p62B; PDB3; ZIP3|
|Sequestosome-1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in Sequestosome-1 result in sporadic and familial Paget disease of bone.|
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Western blot - SQSTM1 / p62 antibody [EPR4844]; All lanes : Anti-SQSTM1 / p62 antibody [EPR4844].Lane 1 : MCF-7.Lane 2 : HeLa.Lane 3 : SKBR-3.Lane 4 : 293T.Lysates/proteins at 10 µg per lane.Observed band size : 62 kDa .
Immunocytochemistry/ Immunofluorescence - SQSTM1 / p62 antibody [EPR4844]; TA307334 staining of SQSTM1/p62 by immunofluorescence
Flow Cytometry - Anti-SQSTM1 / p62 antibody [EPR4844]; Overlay histogram showing HeLa cells stained with TA307334 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.