Anti-SPR Antibody, clone 1F4

Also for SPR (NM_003124)

OriGene Data

Immunogen	Full length human recombinant protein of human SPR (NP_003115) produced in HEK293T cell.
Clone Name	clone 1F4	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR)
Alternative Name	SDR38C1
Database Link	NP_003115
Function	This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.

IF Image

Anti-SPR mouse monoclonal antibody (TA501984) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR(RC205679).

FC Image

HEK293T cells transfected with either RC205679 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SPR antibody(TA501984), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-SPR antibody(TA501984),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-SPR antibody(TA501984),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-SPR TrueMAB Antibody clone 1F4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data