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Home Antibody All anti-SPP1 antibodies

Anti-SPP1 Antibody EPR3688

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307687
  • Rabbit monoclonal antibody against SPP1(clone EPR3688)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC424632) , 20ug
100ul 325 In Stock
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WB(1)
IHC(6)
IF(1)
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Also for SPP1 (NM_000582)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SPP1 was used as an immunogen.
Clone NameEPR3688 IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsFC: 1:40, ICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1000 - 10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2
Alternative NameBNSP; BSPI; ETA-1; OPN
Database LinkNP_000573
FunctionPhosphoprotein 1 (SPP1 or Osteopontin 1) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44. It has been shown to be multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, involve in development and regeneration of skeletal muscle and control of tumor cell phenotype (1). Osteopontin is synthesized by preosteoblasts, osteoblasts and osteocytes, is secreted into osteoid and is incorporated into bone. In addition, to bone cells, osteopontin is synthesized by extraosseous cells in the inner ear, brain, kidney and deciduum and placenta, It is also synthesized by odontoblasts, certain bone marrow cells and hypertrophic chondrocytes (2).
Related Pathway
Focal Adhesion
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Osteopontin antibody [EPR3688]; All lanes : Anti-Osteopontin antibody [EPR3688] at 1/1000 dilution.Lane 1 : MOLT-4 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 35 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Osteopontin antibody [EPR3688]; 1/100 TA307687 staining Osteopontin in formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue by immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR3688]; TA307687 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR3688]; TA307687 showing positive staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR3688]; TA307687 showing positive staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Osteopontin antibody [EPR3688](TA307687); TA307687 showing positive staining in Renal cell carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Osteopontin antibody [EPR3688](TA307687); TA307687 showing positive staining in Stomach adenocarcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Osteopontin antibody [EPR3688]; 1/100 TA307687 staining Osteopontin in Human HeLa cells by Immunofluorescence.

 

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