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Anti-SPN Antibody eBioR2/60


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA320466 Rat Anti-Mouse CD43 Purified (100 ug) 100ug $120 3-7 Days
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OriGene Data

Clone NameeBioR2/60 Isotype
Species ReactivityMouse Concentration0.5-1mg/ml (lot dependent)
Guaranteed Application *FC Suggested DilutionsFlow, IP, WB
BufferAqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Purification Affinity purified

Reference Data

Target NameMus musculus sialophorin (Spn), transcript variant 1
Alternative NameA630014B01Rik; Cd43; Galgp; Ly-48; Ly48
Database LinkNP_033285
Entrez Gene 20737 Mouse
FunctionThe eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

FC Image
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE . Cells in the lymphocyte gate were used for analysis.


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