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Anti-SPG7 TRUEMAB Antibody Clone 1C12
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SPG7 (NM_003119)
|Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) prodduced in E.coli.|
|WB, IF, FC
||WB 1:500~2000, IF 1:50~100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant 1|
|CAR; CMAR; PGN; SPG5C|
|This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (RC206340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Anti-SPG7 mouse monoclonal antibody (TA504420) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7(RC206340).
Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody (TA504420).
HEK293T cells transfected with either RC206340 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SPG7 antibody(TA504420), and then analyzed by flow cytometry.