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Anti-SOX2 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for SOX2 (NM_003106)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)|
Entrez Gene 6657 Human
Entrez Gene 20674 Mouse
|SRY (sex determining region Y)-box 2. Entrez Protein NP_003097. It is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).|
Wnt Signaling Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX2 (RC200757, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: SOX2 Antibody - Cell lysate from 293 over expressing Sox2 was subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-Sox2 antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to Sox2. Data courtesy of a SDI customer.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX2 rabbit polyclonal antibody. (TA590150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-SOX2 rabbit polyclonal antibody. (TA590150)