Home Antibody All anti-SOX2 antibodies
Also for SOX2 (NM_003106)
|This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human SOX2.|
|Human (Predicted: Mouse, Zebrafish, Chicken, Xenopus)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)|
Entrez Gene 6657 Human
Entrez Gene 20674 Mouse
|The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.|
Wnt Signaling Pathway
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Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35ug/lane). SOX2(arrow) was detected using the purified Pab.
Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (Cat.#TA302026). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (Cat.#TA302026), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence analysis of SOX2 Antibody (N-term) (Cat.#TA302026) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.