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Anti-SOX17 TRUEMAB Antibody Clone OTI3B10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SOX17 (NM_022454)
|Human recombinant protein fragment corresponding to amino acids 177-414 of human SOX17 (NP_071899) produced in E.coli.|
|WB, IHC, IF, FC
||WB 1:5000, IHC 1:50, IF 1:50~100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens SRY (sex determining region Y)-box 17 (SOX17)|
Entrez Gene 64321 Human
|SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins.|
|Transcription Factors Wnt signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-SOX17 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500044)
Anti-SOX17 mouse monoclonal antibody (TA500044) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17(RC220888).
Immunofluorescent staining of HepG2 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
Immunofluorescent staining of HT29 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
Figure from citation: Immunofluorescence of SOX17 protein level by using anti-SOX17 antibody in human KhES-3 cells. View Citation
HEK293T cells transfected with either RC220888 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOX17 antibody(TA500044), and then analyzed by flow cytometry.