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Anti-SNCA Antibody EP1646Y
Also for SNCA (NM_000345)
|A synthetic peptide corresponding to residues on the N-terminus of human Alpha-synuclein was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||FC: 1:1000; WB: 1:50000; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-Fr,IHC-P or IP.
|Homo sapiens synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1|
|NACP; PARK1; PARK4; PD1|
|Alpha-synuclein is expressed predominantly in brain, where it is concentrated in presynaptic nerve terminals (1). The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Phosphorylation of alpha-synuclein at Ser 129 promoted fibril formation in vitro and these results highlight the importance of phosphorylation of filamentous proteins in the pathogenesis of neurodegenerative disorders (2). Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. It is hypothesized that parkin and alpha-synculein interact functionally, namely, that parkin ubiquitinates alpha-synuclein normally and that this process is altered in autosomal recessive PD (3). |
EGFR1 Signaling Pathway
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Western blot - alpha Synuclein antibody [EP1646Y]; Anti-alpha Synuclein antibody [EP1646Y] at 1/50000 dilution + Fetal brain lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 18 kDa .
Immunocytochemistry/ Immunofluorescence - alpha Synuclein antibody [EP1646Y]; Immunofluorescent staining of PC12 cells using TA300914 (1:50).
Immunocytochemistry/ Immunofluorescence - Anti-alpha Synuclein antibody [EP1646Y]; Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha Synuclein with TA300914.Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4Â°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
Flow Cytometry - Anti-alpha Synuclein antibody [EP1646Y]; Overlay histogram showing SH-SY5Y cells stained with TA300914 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.