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Home Antibody All anti-SNAP25 antibodies

Anti-SNAP25 TRUEMAB Antibody Clone OTI4B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502970
  • SNAP25 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418912) , 20ug Explanation
100ul $325 In Stock
CF502970
  • Carrier-free (BSA/glycerol-free) SNAP25 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(5)
IF(1)
FC(2)
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Also for SNAP25 (NM_003081)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.
Clone NameClone OTI4B5 IsotypeIgG2a
Species ReactivityHuman Concentration1.1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 23.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1
Alternative NamebA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25
Database LinkNP_003072
Entrez Gene 6616 Human
FunctionSynaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq].
Related PathwayDruggable Genome SNARE interactions in vesicular transport

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (RC202068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970)
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Anti-SNAP25 mouse monoclonal antibody (TA502970) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25(RC202068).
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HEK293T cells transfected with either RC202068 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SNAP25 antibody(TA502970), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-SNAP25 antibody(TA502970),(Red), compared to a nonspecific negative control antibody,(Blue).
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