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Home Antibody All anti-SMO antibodies
UltraMAB

Anti-SMO GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590595
  • Rabbit Polyclonal Smoothened Antibody
50ug 325 In Stock
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WB(2)
IHC(3)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein (NM_005631).
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Mouse Concentration1.1mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens smoothened, frizzled class receptor (SMO)
Alternative NameFZD11; Gx; SMOH
Database LinkNP_005622
FunctionThe protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
Related Pathway
Hedgehog signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMO (RC203451, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMO.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMO polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA Image
ELISA: Smoothened Antibody
IHC Image
Immunohistochemistry: Smoothened Antibody - Immunohistochemical staining for smoothened homolog precursor (SMO) in human testis. In malignant tissues, a moderate cytoplasmic staining was found in human prostate cancer (B). These images are the courtesy of the Human Proteome Resource (HPR).
IHC Image
Immunohistochemistry: Smoothened Antibody - Immunohistochemical staining for smoothened homolog precursor (SMO) in human testis. Ducti seminiferus of the testis stained strongly positive in a cytoplasmic fashion, while a subset of Leydig cells displayed strong cytoplasmic and membranous staining. These images are the courtesy of the Human Proteome Resource (HPR).
IHC Image
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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