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Anti-SMO GAT Antibody Polyclonal
Generated by Genomic Immunization
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein (NM_005631).|
|Human , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens smoothened, frizzled class receptor (SMO)|
|FZD11; Gx; SMOH|
|The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.|
Hedgehog signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMO (RC203451, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMO.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMO polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: Smoothened Antibody - Immunohistochemical staining for smoothened homolog precursor (SMO) in human testis. In malignant tissues, a moderate cytoplasmic staining was found in human prostate cancer (B). These images are the courtesy of the Human Proteome Resource (HPR).
Immunohistochemistry: Smoothened Antibody - Immunohistochemical staining for smoothened homolog precursor (SMO) in human testis. Ducti seminiferus of the testis stained strongly positive in a cytoplasmic fashion, while a subset of Leydig cells displayed strong cytoplasmic and membranous staining. These images are the courtesy of the Human Proteome Resource (HPR).
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.