Home All anti-SMC1A antibodies
Anti-SMC1A Phospho Antibody EP2858Y
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Also for SMC1A (NM_006306)
|A phospho specific peptide corresponding to residues surrounding serine 966 of human SMC1 was used as an immunogen. This antibody detects SMC1 phosphorylated on Serine 966.|
||WB: 1:5000~10,000; ICC: 1:250
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens structural maintenance of chromosomes 1A (SMC1A)|
|CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB|
|Structural maintenance of chromosome proteins (SMC) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. It has been reported that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner (1). ATM phosphorylates Smc1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM substrates Nbs1 and Brca1 (2). |
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Western blot analysis on HeLa cell lysates using anti-Phospho-SMC1 (pS957), 1:1000 dilution. Cells were either (A) untreated (B) treated with hydroxyurea.
Immunofluorescent staining of HeLa cells using anti-SMC1.