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Home Antibody All anti-SMC1A antibodies

Anti-SMC1A PHOSPHO Antibody EPR2857Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301250
  • Rabbit Monoclonal Antibody against SMC1A (Clone EPR2857Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(2)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 957 of human SMC1 was used as an immunogen. This antibody detects SMC1 phosphorylated on ser957.
Clone NameEPR2857Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500 - 1:1000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens structural maintenance of chromosomes 1A (SMC1A), transcript variant 1
Alternative NameCDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB
Database LinkNP_006297
Entrez Gene 8243 Human
FunctionStructural maintenance of chromosome proteins (SMC) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. It has been reported that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner (1). ATM phosphorylates Smc1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM substrates Nbs1 and Brca1 (2).
Related PathwayDruggable Genome Cell cycleOocyte meiosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SMC1 (phospho S957) antibody [EPR2857Y]; All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] at 1/1000 dilution.Lane 1 : Hela cells lysate, untreated.Lane 2 : Hela cells lysate, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 143 kDa.Observed band size : 160 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y]; TA301250 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y]; TA301250 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.

 

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