Invalid object name 'antibody_LocusMapping'. Anti-SMAD5 Antibody, EP728(2)AY - OriGene
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Anti-SMAD5 PHOSPHO Antibody EP728(2)AY

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301089
  • Rabbit Monoclonal Antibody against SMAD5 (Clone EP728(2)AY) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IF(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.
Clone NameEP728(2)AY IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500; ICC/IF: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens SMAD family member 5 (SMAD5), transcript variant 1
Alternative NameDWFC; JV5-1; MADH5
Database LinkNP_005894
FunctionSmad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.
Related Pathway
TGF Beta Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; All lanes : Anti-SMAD5 (phospho S463 + S465) [EP728(2)AY] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with BMP-4.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 52 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; Immunofluorescent staining of HeLa cells using TA301089 at a 1/50-1/100 dilution.

 

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