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Anti-SMAD4 Antibody EP618Y


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Smad4 (C-term)(EP618Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401648) , 20ug Explanation
100ul 325 In Stock
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Also for SMAD4 (NM_005359)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen.
Clone NameEP618Y IsotypeRabbit IgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1,000-5,000; IHC: 1:100-250; ICC: 1:100-250; FC: 1:20; IP: 1:30
Buffer50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Reference Data

Target NameHomo sapiens SMAD family member 4 (SMAD4)
Alternative NameDPC4; JIP; MADH4; MYHRS
Database LinkNP_005350
Entrez Gene 4089 Human
Entrez Gene 17128 Mouse
Entrez Gene 50554 Rat
Related PathwayTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad4 antibody [EP618Y]; Anti-Smad4 antibody [EP618Y] at 1/5000 dilution + SHSY5Y cell lysate at 10 ug.Predicted band size : 65 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smad4 antibody [EP618Y]; Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TA303589 diluted 1:100
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y]; TA303589 staining Smad4 in Rat femur tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 20 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 20°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EP618Y]; TA303589 staining Smad4 in theA549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde andpermeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugatedGoat anti-rabbit polyclonal (1/200)was used as the secondary antibody.
IF Image
Immunofluorescence - Smad4 antibody [EP618Y]; Immunofluorescent staining of HeLa cells using ab 40759 diluted 1:100
FC Image
Flow Cytometry - Smad4 antibody [EP618Y]; Flow cytometric analysis of permeabilized A431 cells using anti-Smad4 RabMAb (red) or a rabbit IgG (negative) (green).


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