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Anti-SMAD4 Antibody EP618Y
Also for SMAD4 (NM_005359)
|A synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1,000-5,000; IHC: 1:100-250; ICC: 1:100-250; FC: 1:20; IP: 1:30
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
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Western blot - Smad4 antibody [EP618Y]; Anti-Smad4 antibody [EP618Y] at 1/5000 dilution + SHSY5Y cell lysate at 10 ug.Predicted band size : 65 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Smad4 antibody [EP618Y]; Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TA303589 diluted 1:100
IHC - Anti-Smad4 antibody; TA303589 staining Smad4 in Rat femur tissue sections by IHC (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 20 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 20°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EP618Y]; TA303589 staining Smad4 in theA549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde andpermeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25Â°C. A TRITC-conjugatedGoat anti-rabbit polyclonal (1/200)was used as the secondary antibody.
Immunofluorescence - Smad4 antibody [EP618Y]; Immunofluorescent staining of HeLa cells using ab 40759 diluted 1:100
Flow Cytometry - Smad4 antibody [EP618Y]; Flow cytometric analysis of permeabilized A431 cells using anti-Smad4 RabMAb (red) or a rabbit IgG (negative) (green).