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Home Antibody All anti-SMAD3 antibodies

Anti-SMAD3 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA325124
  • Rabbit polyclonal SMAD3-S208 Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401784) , 20ug
400ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for SMAD3 (NM_005902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Alternative NameHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Database LinkNP_005893
Function
Related Pathway
Adherens junction
Adipogenesis
Cell cycle
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
SMAD3 Antibody (S208) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
IHC Image
SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
IF Image
Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
FC Image
SMAD3-S208 Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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