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Home Antibody All anti-SMAD3 antibodies

Anti-SMAD3 Antibody EP568Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300653
  • Rabbit Monoclonal Antibody against SMAD3 (Clone EP568Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401784) , 20ug
100ul 325 In Stock
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WB(1)
IHC(6)
FC(1)
ELISA(1)
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Also for SMAD3 (NM_005902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to the internal region of human Smad3 was used as an immunogen.
Clone NameEP568Y IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ELISA, IHC, FC Suggested DilutionsWB: 1:5000; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; Sandwich ELISA: Use a concentration of 0.5 ug/ml; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Alternative NameHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Database LinkNP_005893
FunctionSmad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-ß/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-ß), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-ß receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- ß target genes. Interaction of Smad3 with Akt, can initiate TGF- ß induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis (4).
Related Pathway
Adherens junction
Adipogenesis
Cell cycle
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - Smad3 antibody [EP568Y]; Anti-Smad3 antibody [EP568Y] at 1/5000 dilution + Jurkat cell lysate at 10 µg.Predicted band size : 48 kDa.Observed band size : 55 kDa .
ELISA Image
Sandwich ELISA - Smad3 antibody [EP568Y]; Standard Curve for Smad3 (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 5ug/ml and Detector Antibody at 0.5ug/ml.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Smad3 antibody [EP568Y]; TA300653 at a 1:100 dilution staining Smad3 in human prostate carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Gastric adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Lung adenocarcinoma tissue.
FC Image
Flow Cytometry-Anti-Smad3 antibody [EP568Y](TA300653); Overlay histogram showing HCT116 cells stained with TA300653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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