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Home Antibody All anti-SMAD3 antibodies

Anti-SMAD3 Antibody EP568Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300653
  • Rabbit Monoclonal Antibody against SMAD3 (Clone EP568Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401784) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(6)
FC(1)
ELISA(1)
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Also for SMAD3 (NM_005902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to the internal region of human Smad3 was used as an immunogen.
Clone NameEP568Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ELISA, IHC, FC Suggested DilutionsWB: 1:5000; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; Sandwich ELISA: Use a concentration of 0.5 ug/ml; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Alternative NameHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Database LinkNP_005893
Entrez Gene 4088 Human
Entrez Gene 17127 Mouse
Entrez Gene 25631 Rat
FunctionSmad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-ß/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-ß), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-ß receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- ß target genes. Interaction of Smad3 with Akt, can initiate TGF- ß induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis (4).
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad3 antibody [EP568Y]; Anti-Smad3 antibody [EP568Y] at 1/5000 dilution + Jurkat cell lysate at 10 ug.Predicted band size : 48 kDa.Observed band size : 55 kDa .
ELISA Image
Sandwich ELISA - Smad3 antibody [EP568Y]; Standard Curve for Smad3 (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 5ug/ml and Detector Antibody at 0.5ug/ml.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Smad3 antibody [EP568Y]; TA300653 at a 1:100 dilution staining Smad3 in human prostate carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Gastric adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Smad3 antibody [EP568Y](TA300653); TA300653 showing positive staining in Lung adenocarcinoma tissue.
FC Image
Flow Cytometry-Anti-Smad3 antibody [EP568Y](TA300653); Overlay histogram showing HCT116 cells stained with TA300653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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