Anti-SMAD2 Antibody, clone 4G7

Also for SMAD2 (NM_005901)

OriGene Data

Immunogen	Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.
Clone Name	clone 4G7	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1
Alternative Name	hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database Link	NP_005892
Function	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].
Related Pathway	Adherens junction Cell cycle EGFR1 Signaling Pathway TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD2 (RC204604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD2.

IF Image

Anti-SMAD2 mouse monoclonal antibody (TA501777) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMAD2(RC204604).

FC Image

HEK293T cells transfected with either RC204604 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SMAD2 antibody(TA501777), and then analyzed by flow cytometry.

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Anti-SMAD2 TrueMAB Antibody clone 4G7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data