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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324933
  • Rabbit polyclonal SMAD2 Antibody (T220)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400372) , 20ug Explanation
400ul 325 3-7 Days
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WB(1)
IF(1)
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Also for SMAD2 (NM_001003652)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from human SMAD2.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat, Zebrafish, Bovine) Concentration0.5 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsIF: 1:200, WB: 1:1000
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 2
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_001003652
Entrez Gene 4087 Human
Entrez Gene 17126 Mouse
Entrez Gene 29357 Rat
Function
Related Pathway
Adherens junction
Cell cycle
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of SMAD2 Antibody (T220) (Cat.# TA324933) in NCI-H460 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.
IF Image
Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324933 SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.

 

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