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Also for SMAD2 (NM_005901)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Smad2 (Ab-220) antibody detects endogenous levels of total Smad2 protein
|Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1|
|hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2|
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot analysis of extracts from RAW264.7 cells and 293 cells, using Smad2 (Ab-220) antibody.The lane on the right is treated with the synthesized peptide.