Home Antibody All anti-SMAD2 antibodies
Anti-SMAD2 Antibody EP784Y
Also for SMAD2 (NM_005901)
|A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.|
|Rat, Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; IP: 1:50; WB: 1:10000; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Smad2 antibody [EP784Y]; Anti-Smad2 antibody [EP784Y] at 1/500000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP784Y]; TA300654 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.
Flow Cytometry - Anti-Smad2 antibody [EP784Y]; Overlay histogram showing PC3 cells stained with TA300654 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.