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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody EP567Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300564
  • Rabbit Monoclonal Antibody against SMAD2 (Clone EP567Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401783) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
IHC(1)
FC(1)
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Also for SMAD2 (NM_005901)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.
Clone NameEP567Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; FC: 1:20 - 1:100
Predicted MW Explanation 58 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 1
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_005892
Entrez Gene 4087 Human
Entrez Gene 17126 Mouse
Entrez Gene 29357 Rat
FunctionSmad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad2 antibody [EP567Y]; Anti-Smad2 antibody [EP567Y] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
WB Image
Western blot - Smad2 antibody [EP567Y]; All lanes : Anti-Smad2 antibody [EP567Y] at 1/3000 dilution.Lane 1 : HGL-5 cells - no additives.Lane 2 : Cells + activin A.Lane 3 : Cells + cyclophilin siRNA Control.Lane 4 : Cells + activin A + cyclophilin.Lane 5 : non-targeting, negative control for siRNA.Lane 6 : Cells + activin A, negative control for siRNA.Lane 7 : Cells + siRNA Smartpool.Lane 8 : Cells + activin A + siRNA Smartpool.Lane 9 : Jurkat cells.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/50000 dilution.Performed under reducing conditions.Predicted band size : 58 kDa.Observed band size : 58 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP567Y]; TA300564, at a 1/50 dilution,staining human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
FC Image
Flow Cytometry - Smad2 antibody [EP567Y]; Overlay histogram showing PC3 cells stained with TA300564 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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