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Anti-SMAD2 Antibody EP567Y
Also for SMAD2 (NM_005901)
|A synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; FC: 1:20 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IP.
|Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1|
|hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2|
|Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5). |
EGFR1 Signaling Pathway
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Western blot - Smad2 antibody [EP567Y]; Anti-Smad2 antibody [EP567Y] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Western blot - Smad2 antibody [EP567Y]; All lanes : Anti-Smad2 antibody [EP567Y] at 1/3000 dilution.Lane 1 : HGL-5 cells - no additives.Lane 2 : Cells + activin A.Lane 3 : Cells + cyclophilin siRNA Control.Lane 4 : Cells + activin A + cyclophilin.Lane 5 : non-targeting, negative control for siRNA.Lane 6 : Cells + activin A, negative control for siRNA.Lane 7 : Cells + siRNA Smartpool.Lane 8 : Cells + activin A + siRNA Smartpool.Lane 9 : Jurkat cells.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/50000 dilution.Performed under reducing conditions.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP567Y]; TA300564, at a 1/50 dilution,staining human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry - Smad2 antibody [EP567Y]; Overlay histogram showing PC3 cells stained with TA300564 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.