EGFR Rat Monoclonal Antibody [Clone ID: ICR10]

CAT#: SM1334P

EGFR rat monoclonal antibody, clone ICR10, Purified

Conjugation: Unconjugated FITC


USD 569.00

5 Days*

Size
    • 200 ug

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Specifications

Product Data
Clone Name ICR10
Applications FC, IHC, IP, WB
Recommended Dilution Immunoprecipitation.
Western blotting.
Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen sections.
Recommended positive control: Breast carcinoma.
Reactivities Human
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Extracellular domain of human Epidermal Growth Factor Receptor (EGFR) from head and neck carcinoma.
Specificity This antibody recognises the epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. This antibody binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.
Formulation PBS, pH7.4 containing 0.09% Sodium Azide
State: Purified
State: Liquid purified IgG
Concentration lot specific
Purification Ion exchange chromatography
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.