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Anti-SLC4A1 PHOSPHO Antibody EPR1427Y
Also for SLC4A1 (NM_000342)
|A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human AE1 was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000; ICC: 1:100 - 250; FC: 1:20;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens solute carrier family 4 (anion exchanger), member 1 (Diego blood group) (SLC4A1)|
|AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SW; WD; WD1; WR|
|The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr359 and Tyr404 by Lyn in erythrocytes. Phosphorylation of these residues is necessary for correct localization and recycling of kidney AE1 to the basolateral membrane (2).|
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Western blot - Band 3 (phospho Y359) antibody [EPR1427Y]; All lanes : Anti-Band 3 (phospho Y359) antibody [EPR1427Y] at 1/50000 dilution.Lane 1 : Red blood cell lysate, untreated.Lane 2 : Red blood cell lysate treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 102 kDa.