Anti-SLC2A5 TrueMAB Antibody clone 12F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

SKU	Description	Amount	Price	Availability*
TA500575	SLC2A5 (GLUT5) mouse monoclonal antibody, clone 12F2 FREE positive control: HEK293T cell transient overexpression lysate (LC401061) , 20ug	100ul 30ul	$325	In Stock
CF500575	Carrier-free (BSA/glycerol-free) SLC2A5 mouse monoclonal antibody, clone 12F2	100ug	$450	3 Days
TA802519S	Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9	30ul	$100 $50*	In Stock

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

WB(1)

IP(1)

IF(1)

FC(1)

Also for SLC2A5 (NM_003039)

OriGene Data

Immunogen	Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
Clone Name	clone 12F2	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, IP, FC	Suggested Dilutions	WB 1:1000, IF 1:50, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1
Alternative Name	GLUT-5; GLUT5
Database Link	NP_003030
Function
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.

IF Image

Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).

IP Image

Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC200418 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC2A5 antibody(TA500575), and then analyzed by flow cytometry.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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