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Anti-SLC2A5 TrueMAB Antibody clone 12F2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for SLC2A5 (NM_003039)
|Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, IP, FC
||WB 1:1000, IF 1:50, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.
Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).
Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC200418 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC2A5 antibody(TA500575), and then analyzed by flow cytometry.