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Home Antibody All anti-SLC2A1 antibodies

Anti-SLC2A1 Antibody EPR3915

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307154
  • Rabbit monoclonal antibody against Glut-1(clone EPR3915)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416593) , 20ug
100ul $325 In Stock
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WB(1)
IHC(9)
FC(1)
Assay(1)
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Also for SLC2A1 (NM_006516)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Glut-1 was used as an immunogen.
Clone NameEPR3915 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500; FC: 1:100 - 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1)
Alternative NameDYT17; DYT18; DYT9; EIG12; GLUT; GLUT1; GLUT1DS; HTLVR; PED
Database LinkNP_006507
FunctionGlucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier (1). It is expressed in high density on the membranes of human erythrocytes and on the brain capillaries that comprise the blood-brain barrier (2). Glut-1 can transport a wide range of aldoses, including both pentoses and hexoses (3). Mutations in Glut-1 have been found in a family with paroxysmal exertion-induced dyskinesia (1). Isoforms exist from 40 to 60 kDa, depending on the tissue and post-translational modifications.
Related Pathway

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WB Image
Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915]; All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] at 1/1000 dilution.Lane 1 : Jurkat lysate.Lane 2 : Mouse brain lysate.Lane 3 : Human fetal brain lysate.Lane 4 : 3T3L1 lysate.Lane 5 : Human fetal liver lysate.Lane 6 : HepG2 lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 54 kDa.
Assay Image
Other-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915]; TA307154 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915]; TA307154 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing positive staining in Normal breast tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing positive staining in Kidney carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing negative staining in Skeletal muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing positive staining in Urinary bladder transitional carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glucose Transporter GLUT1 antibody [EPR3915](TA307154); TA307154 showing negative staining in Normal heart tissue.
FC Image
Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [EPR3915]; Overlay histogram showing HeLa cells stained with TA307154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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