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Anti-SIRT5 TRUEMAB Antibody clone 1G9
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SIRT5 (NM_012241)
|Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell. |
|| WB 1:2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens sirtuin 5 (SIRT5), transcript variant 1|
Entrez Gene 23408 Human
|This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]. |
|Transcription FactorsDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503279), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SIRT5 antibody(TA503279),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SIRT5 antibody(TA503279),(Red), compared to a nonspecific negative control antibody,(Blue).