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Anti-SHH TRUEMAB Antibody clone 3A2
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH|
||WB: 1:2000, IF (1:100)
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens sonic hedgehog (SHH)|
|HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS|
|SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.|
Hedgehog signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH(RC222175) (RC222175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
Anti-SHH mouse monoclonal antibody (TA500040) immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH(RC222175).