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Anti-SHC1 PHOSPHO Antibody EP419
Also for SHC1 (NM_183001)
|A synthetic peptide corresponding to residues surrounding Tyrosine 239/240 of human SHC1 was used as an immunogen. The antibody only detects SHC1 phosphorylated at Tyrosine 239/240.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 1|
|The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHC isoforms are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5). |
EGFR1 Signaling Pathway
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Western blot - SHC (phospho Y239 + Y240) antibody [EP419]; All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] at 1/500 dilution.Lane 1 : 293T cell lysates, untreated.Lane 2 : 293T cell lysates, treated with EGF.Lysates/proteins at 10 µg per lane.Predicted band size : 63 kDa.