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Anti-SF1 PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human SF1 around the phosphorylation site of serine 82 (S-P-SP-P-E).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|SF1 (Phospho-Ser82) antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
|Homo sapiens splicing factor 1 (SF1), transcript variant 3|
|BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162|
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Western blot analysis of extracts from HUVEC cells, treated with anisomycin (25ug/ml, 30mins), using SF1 (Phospho-Ser82) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibodyThe picture on the right is treated with the synthesized peptide.