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Anti-SENP1 Antibody EPR3844
Also for SENP1 (NM_014554)
|A synthetic peptide corresponding to residues in human SENP1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: 1:500; WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens SUMO1/sentrin specific peptidase 1 (SENP1)|
|Sentrin-specific protease 1 (SENP1) is a protease that catalyzes several essential functions in the small ubiquitin-like modifier protein (SUMO) pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms; deconjugation of SUMO1, SUMO2 and SUMO3 from protein targets; and depolymerization of SUMO’s conjugated within polymeric chains (1). Therefore, SENP1 regulates the sumoylation state of target proteins in cells during post-translational modification (2). This process involves cellular functions such as gene transcription, nucleocytoplasmic transport, and signal transduction (3). SENP1 also contains conserved C-terminal domains with unique specificities that catalyze protease activity (4). It is highly expressed in the testis and is also expressed at lower levels in the thymus, pancreas, and spleen (1).|
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Western blot - SENP1 antibody [EPR3844]; All lanes : Anti-SENP1 antibody [EPR3844] at 1/1000 dilution.Lane 1 : HeLa cell lysates .Lane 2 : HUVEC cell lysates .Lane 3 : Jurkat cell lysates .Lane 4 : Daudi cell lysates.Lane 5 : U87-MG cell lysates .Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 73 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SENP1 antibody [EPR3844]; Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108981
Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844]; ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.