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Home Antibody All anti-SCARB1 antibodies

Anti-SCARB1 Antibody EP1556Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303871
  • Rabbit monoclonal antibody against SR-B1 Scavenge Receptor (EP1556Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401684) , 20ug
100ul $325 In Stock
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WB(2)
FC(1)
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Also for SCARB1 (NM_005505)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human SR-B1 was used as an immunogen.
Clone NameEP1556Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:2000; ICC: 1:50 - 1:100; FC: 1:100; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens scavenger receptor class B, member 1 (SCARB1), transcript variant 1
Alternative NameCD36L1; CLA-1; CLA1; HDLQTL6; SR-BI; SRB1
Database LinkNP_005496
Function
Related Pathway

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WB Image
Western blot - Scavenging Receptor SR-BI antibody [EP1556Y]; Anti-Scavenging Receptor SR-BI antibody [EP1556Y] at 1/2000 dilution + Mouse liver lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 80 kDa .
WB Image
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y]; .developed using the ECL technique.Performed under reducing conditions.
FC Image
Flow Cytometry - Anti-Scavenging Receptor SR-BI antibody [EP1556Y]; Overlay histogram showing Jurkat cells stained with TA303871 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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